The standard pierce bca protein assay detects protein concentrations from 20 to 2,000. The amount of purplecolored product formed during the reaction can be correlated to the amount of protein in the bca protein assay. Prepare pierce bovine serum albumin bsa standards 2,000. Oct 22, 2007 the bca protein assay kit from thermo scientific pierce product no. Bca protein assay kit from pierce provides a simple method for quantifying protein over a large range. Bca protein assay reducing agent compatible standard tube 1252,000 gml bca protein assay standard tube or microplate 202,000 gml enhanced tube 5250 gml micro bcaprotein assay standard tube 0. The micro bca protein assay kit is a specialized version of the popular pierce bca protein assay for determining the protein concentration of dilute samples. The kit enables you to measure protein concentration in samples that contain the disulfide reducing agents. Abcam s bca protein assay kit reducing agent compatible microplate ab207003 measures total protein concentration of pure proteins, extracts or lysates in the presence of reducing agents.
Pierce bca protein assay kit is a twocomponent, highprecision, detergentcompatible protein assay for determination of protein concentration. The bca protein assay kit reducing agent compatible bcarac provides all of the advantages of the original bca assay, plus compatibility with disulfide reducing agents at concentrations routinely used in protein sample buffers. To determine the total protein concentration of a sample a bsa standard curve has to be set. The thermo scientific pierce bca protein assay kit is a detergentcompatible formulation based on bicinchoninic acid bca for the colorimetric detection and quantitation of total protein. Pierce bca protein assay kit university of arizona. The 384well, flat bottom, nontreated, transparent, sterile polystyrene plates cat. Pierce microplate bca protein assay kit fisher scientific. The pierce bca assay can be used to assess yields in whole cell.
The thermo scientific pierce bca protein assay kit is a twocomponent, highprecision, detergentcompatible assay reagent set to measure a562nm total protein concentration compared to a protein standard. Thermo scientific pierce 660 nm protein assay overview highlights typical response curves 14 bca based protein assays 15 chemistry of the bca protein assay 15 advantages of the bca protein assay 16 disadvantages of the bca protein assay 17 bca, reducing agent compatible 18 bca protein assay 19 micro bca protein assay 20. Pierce bca protein assay kit is a twocomponent, highprecision, detergent compatible protein assay for determination of protein concentration. Thermo scientific pierce bca protein assay kit fisher. The pierce micro bca protein assay reagent kit is a detergentcompatible bicinchoninic acid formulation for the colorimetric detection and quantitation of total protein. Thermo scientific pierce bca protein assay kit protein. The intensity of the blue complex is proportional to the amount of protein in the sample. Pierce rapid gold bca protein assay kit catalog numbers a53225, a53226, and a53227 doc. The micro bicinchoninic acid bca protein assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions and is a modification of the bicinchoninic acid bca protein assay for dilute protein samples 0. I have been using a pierce bca protein assay kit thermo scientific and diluting my samples saliva.
Thermo scientific pierce protein assay technical handbook nugi. Compound buffer whose formulation is described more fully in the follo wing table. Thermo scientific pierce bca protein assay kit bca protein. The micro bca protein assay kit uses concentrated reagents and a protocol that utilizes an extended incubation time at an elevated temperature 60c, test tube procedure only. Which is the best protein assay to determine the concentration in. Incompatible substances amount compatible buffer systems nacetylglucosamine 10 mm in pbs, ph 7. Setting up and running miniprotean tgx precast gels duration. Compare all available bca protein assays features of. The quick start bradford protein assay is a simple and accurate procedure for determining the concentration of protein in solution. The bca assay has a linear concentration range between 2001,000 g of protein per milliliter. Pierce bca protein assay kit, sufficient reagents for 250 testtube or 2500 microplate assays. This is the protocol to be used in the determination of total protein in brain tissue by the pierce bca method. Reagents bicinchoninic acid solution, catalog number b9643 reagent a is a 1,000 ml. This kit was previously called bca protein quantification kit.
The result is an extremely sensitive colorimetric protein assay in a. Bsa bovine serum albumin is used as the standard and samples are. Smith at the pierce chemical company, now part of thermo fisher scientific, is a biochemical assay for determining the total concentration of protein in a solution 0. This bca protein assay kit is a reducing agentcompatible version of our popular pierce bca protein assay. Bca protein assay kit rockford, il 61105 pdf book manual. This method has greater protein to protein variability than the bca assay. Compared to most dyebinding methods, the bca assay is affected much less by protein compositional differences, providing greater protein to protein uniformity. Value when the 660nm assay is run using the ionic detergent compatibility reagent idcr, part no. Bicinchoninic acid bca assay in low volume sciencedirect. I must repeatedly declare that the directphotometric proteinassay without purification is very.
In any protein assay, the best protein to use as a standard is a purified preparation of the protein being assayed. Reducing agent compatible, sufficient reagents to assay 250 samples or standards of 25l each. Results of these compatibility experiments are summarized in tabular form on the following two pages. An adaptaon of the bca protein assay kit, the micro bca kit has been opmized for use with dilute protein samples 0. Quick start bradford protein assay life science research. Pierce bca protein assay kit pdf book manual free download. This site is like a library, you could find million book here by using search box in the.
The bca protein assay kit is a twocomponent, highprecision, detergentcompatible assay reagent set to measure total protein concentration compared to a protein standard. Search results for bca protein assay kit at sigmaaldrich. Pierce microplate bca protein assay kit reducing agent compatible, sufficient reagents for. The pierce bca assay can be used to assess yields in whole cell lysates and affinitycolumn fractions, as well as to monitor protein contamination in industrial applications.
Micro bca protein assay kit pierce micro bicinchoninic acid. An adaptation of pierces bca protein assay reagent kit product no. Measuring the od at 595nm of the protein solution determines the final protein concentration. The thermo scientific pierce bca protein assay kit is a detergent compatible formulation based on bicinchoninic acid bca for the. Bca protein assay reagent bicinchoninic acid youtube. Isobaric mass tagging kits and reagents 41 appendix 44.
We did the pierce bca assay and ran the standard curve and were able to estimate protein concentrations in our samples. The bca assay has a linear concentration range between. An adaptation of pierce s bca protein assay reagent kit product no. Pierce bca reagents provide accurate determination of protein concentration with most sample types encountered in protein research. Download bca protein assay kit rockford, il 61105 book pdf free download link or read online here in pdf. Micro bca protein assay kit is a readytouse, detergentcompatible, western blot related, total protein analysis reagent, used for the quick determination of total protein concentration of dilute protein solutions by measuring absorbance at 562 nm and comparing to a protein standard absorbance vs. Read online pierce bca protein assay kit book pdf free download link book now. Unlike the majority of protein assay kits, ab207003 is compatible with strong reducing agents such as tcep up to 20 mm, dtt up to 10 mm and. Biovisions bca protein assay kit provides a colorimetric detection and quantification of total protein content even in the presence of detergents. Measuring the protein concentration of samples is essential for many protein assays, such as western blotting or zymography.
Micro bca protein assay kit pierce micro bicinchoninic. Measuring protein concentration using the bca protein assay with. Thermo scientific pierce protein assay technical handbook. The pierce bca protein assay is a detergentcompatible formulation based on bicinchoninic acid bca for the colorimetric detection and quantitation of total protein. In test tubes or 96well plates, the relationship between protein concentration and absorbance is nearly linear over a wide working. Introduction the modulus by turner biosystems and the bca protein assay kit from pierce provides a simple method for quantifying protein over a large range. Thermo scientific pierce bca protein assay kit reducing. Read online bca protein assay kit rockford, il 61105 book pdf free download link book now. All books are in clear copy here, and all files are secure so dont worry about it. The bca protein assay is suitable for measuring protein concentration in the range of 0. The bicinchoninic acid assay bca assay, also known as the smith assay, after its inventor, paul k. Download pierce bca protein assay kit book pdf free download link or read online here in pdf.
If precise quantitation of an unknown protein is required, it is advisable to select a protein. Bicinchoninic acid protein assay kit bca1 technical bulletin. The bca protein assay kit is a detergentcompatible formulation based on bicinchoninic acid bca for the colorimetric detection and quantitation of total protein. Selected values for the regular bcarac kit are given in parentheses in the column for the microplate bcarac. Micro bca protein assay kit thermo fisher scientific.
Measuring protein concentration using the bca protein assay. The pierce bca protein assay kit was purchased from thermo scientific waltham, ma and the working reagent wr was prepared as instructed in the users manual. Thermo scientific pierce 660 nm protein assay overview highlights typical response curves 14 bca based protein assays 15 chemistry of the bca protein assay 15 advantages of the bca protein assay 16 disadvantages of the bca protein assay 17. Bca protein assay reagent kit, sufficient reagents for 500 test tube or 5,000 microplate. Pierce bca protein assay kit reducing agent compatible. Compatibility chart for bca kit the amount listed is the maximum amount of material allowed in the protein sample without causing a noticeable interference, when 20 ul protein sample is used for bca assay as described in the standard assay. Pierce bca protein assay kit thermo fisher scientific. Thermo scientific pierce 660 nm protein assay overview highlights typical response curves 14 bcabased protein assays 15 chemistry of the bca protein assay 15 advantages of the bca protein assay 16 disadvantages of the bca protein assay 17 bca, reducing agent compatible 18 bca protein assay 19 micro bca protein assay 20. This method has a fast detection speed and low protein to protein variability in comparison to the bca or coomassie bradford assays. We recommend the use of gloves, lab coats and eye protection. Bicinchoninic acid protein assay kit bca1 technical.
Bca protein assay kit from thermo scientific pierce. Bca bicinchoninic acid bsa bovine serum albumin ci confidence interval dpbs dulbeccos pbs id identification rt room temperature sds safety data sheets. This protocol makes use of thermo scientifics bca protein assay in a 96well tissue culture plate format to measure the amount of protein in small volumes of sample. Piercea protein assay kit bc thermo fisher scientific.
Compared to most dyebinding methods, the bca assay is affected much less by protein compositional differences, providing greater proteintoprotein uniformity. The bicinchoninic acid bca 2 assay is a colorimetric method that is commonly employed to estimate the concentration of protein in a sample. Protocol the following are instructions for the microplate assay format of the pierce bca protein assay kit thermo scientific cat. Jun 19, 2018 bca protein assay reagent bicinchoninic acid duration.
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